lmrA [2019-09-02 11:15:56]

transcriptional regulator ([SW|TetR family]), control of [gene|6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A|qdoI]-[gene|search|yxaH ]and [gene|52D560AA02F0849CB24460A496021560063B2E12|lmrA]-[gene|23483430AE0381406EF2349A18CFAE1F14F9B180|lmrB]

locus: BSU_02680

pI: 5.03

mw: 20.52 kDa

protein length: 188 aa

gene length: 567 bp

function: regulation of lincomycin resistance

product: transcriptional regulator

essential: no

synonyms: lin-2,yccB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]

Gene

Coordinates: 290,132 290,698

The protein

Protein family

• [SW|TetR family]

Paralogous protein(s)

• [protein|47387014B5E89BF218BA94D8C2A570479B1EFD4E|QdoR]

Effectors of protein activity

• flavonoids such as quercetin serve as inducers, binding results in release from DNA [Pubmed|17483215]

Structure

• [PDB|1SGM] ([protein|47387014B5E89BF218BA94D8C2A570479B1EFD4E|QdoR], 56% identity) [pubmed|16475182]

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000222 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|52D560AA02F0849CB24460A496021560063B2E12|lmrA]-[gene|23483430AE0381406EF2349A18CFAE1F14F9B180|lmrB]
  description: [Pubmed|15317768]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12499232], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|52D560AA02F0849CB24460A496021560063B2E12|LmrA]: repression, [Pubmed|15317768], in [regulon|52D560AA02F0849CB24460A496021560063B2E12|LmrA regulon]

  regulation

  • induced by flavonoids such as quercetin ([protein|search|LmrA])[Pubmed|17483215]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE02680 ([gene|52D560AA02F0849CB24460A496021560063B2E12|lmrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATTCCCACCTTACT, downstream forward: _UP4_TAAAAAAAACGACATACTAC
• BKK02680 ([gene|52D560AA02F0849CB24460A496021560063B2E12|lmrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATTCCCACCTTACT, downstream forward: _UP4_TAAAAAAAACGACATACTAC

References

Reviews

• 24006471,25209494

Original publications

• 15317768,17483215,12499232,21815947,16475182